The Pseudomonas chlororaphis PCL1391 Sigma Regulator psrA Represses the Production of the Antifungal Metabolite Phenazine-1-Carboxamide

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Regulatory roles of psrA and rpoS in phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis PCL1391.

Production of the secondary metabolite phenazine-1-carboxamide (PCN) by Pseudomonas chlororaphis PCL1391 is crucial for biocontrol activity against the phytopathogen Fusarium oxysporum f. sp. radicis lycopersici on tomato. Regulation of PCN production involves the two-component signalling system GacS/GacA, the quorum-sensing system PhzI/PhzR and the regulator PsrA. This paper reports that a fun...

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Influence of environmental conditions on the production of phenazine-1-carboxamide by Pseudomonas chlororaphis PCL1391.

Pseudomonas chlororaphis PCL1391 produces the secondary metabolite phenazine-1-carboxamide (PCN), which is an antifungal metabolite required for biocontrol activity of the strain. Identification of conditions involved in PCN production showed that some carbon sources and all amino acids tested promote PCN levels. Decreasing the pH from 7 to 6 or decreasing the growth temperature from 21 to 16 d...

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Genetic regulation of phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis strain PCL1391

Among Gram-negative bacteria, pseudomonads produce a wide variety of secondary metabolites, including simple organic molecules such as hydrogen cyanide, enzymes such as chitinases, elastase and exoproteases, and antibiotics such as 2,4-diacetyl-phloroglucinol (Phl) and phenazines. Many of these metabolites are involved in interactions with other organisms, including pathogenesis of plants, anim...

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MPMI Vol

The rhizobacterium Pseudomonas chlororaphis PCL1391 produces the antifungal metabolite phenazine-1-carboxamide (PCN), which is a crucial trait in its competition with the phytopathogenic fungus Fusarium oxysporum f. sp. radicis-lycopersici in the rhizosphere. The expression of the PCN biosynthetic gene cluster in PCL1391 is population density–dependent and is regulated by the quorum-sensing gen...

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Phenazine-1-carboxamide production in the biocontrol strain Pseudomonas chlororaphis PCL1391 is regulated by multiple factors secreted into the growth medium.

Pseudomonas chlororaphis PCL1391 controls tomato foot and root rot caused by Fusarium oxysporum f. sp. radicis-lycopersici. The production of phenazine-1-carboxamide (PCN) is crucial for this biocontrol activity. In vitro production of PCN is observed only at high-population densities, suggesting that production is under the regulation of quorum sensing. The main autoinducer molecule produced b...

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ژورنال

عنوان ژورنال: Molecular Plant-Microbe Interactions®

سال: 2005

ISSN: 0894-0282,1943-7706

DOI: 10.1094/mpmi-18-0244